Description
We describe procedures for the preparation and delivery of membrane protein microcrystals in lipidic cubic phase for serial crystallography at X-ray free-electron lasers and synchrotron sources. These protocols can also be applied for incorporation and delivery of soluble protein microcrystals, leading to substantially reduced sample consumption compared to liquid injection.
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Title
- Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography
Contributors
- Ishchenko, Andrii (Author)
- Cherezov, Vadim (Author)
- Liu, Wei (Author)
- College of Liberal Arts and Sciences (Contributor)
Date Created
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2016-09-20
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Identifier
- Digital object identifier: 10.3791/54463
- Identifier TypeInternational standard serial numberIdentifier Value1940-087X
Note
- The final version of this article, as published in Journal of Visualized Experiments, can be viewed online at: https://www.jove.com/video/54463/preparation-delivery-protein-microcrystals-lipidic-cubic-phase-for, opens in a new window
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Ishchenko, A., Cherezov, V., & Liu, W. (2016). Preparation and Delivery of Protein Microcrystals in Lipidic Cubic Phase for Serial Femtosecond Crystallography. Journal of Visualized Experiments, (115). doi:10.3791/54463