ASU Scholarship Showcase

Permanent Link Feedback

Series
  • FRONTIERS IN MICROBIOLOGY
Date Range
2013 2016

Chloroflexus aurantiacus is an anoxygenic phototrophic bacterium. Its unique CO[subscript 2] fixation pathway and primitive light-harvesting antenna complexes have attracted extensive research attentions. In this work, we investigated the photoheterotrophic growth of C. aurantiacus J-10-fl using acetate [at 55°C and without H[subscript 2(g)]]. The results indicate that glycine can promote anaerobic biomass production in a minimal medium by threefold to fivefold. Via 13C-metabolite analysis, we observed that glycine was involved in serine synthesis. Instead of being used as a major carbon source, glycine was degraded to produce C1 units and NAD(P)H. Tracer experiments also suggest that photoheterotrophic cultures growing with ...

Contributors
He, Lian, Wang, Yaya, You, Le, et al.
Created Date
2015-12-22

Synthetic Biology promises low-cost, exponentially scalable products and global health solutions in the form of self-replicating organisms, or “living devices.” As these promises are realized, proof-of-concept systems will gradually migrate from tightly regulated laboratory or industrial environments into private spaces as, for instance, probiotic health products, food, and even do-it-yourself bioengineered systems. What additional steps, if any, should be taken before releasing engineered self-replicating organisms into a broader user space? In this review, we explain how studies of genetically modified organisms lay groundwork for the future landscape of biosafety. Early in the design process, biological engineers are anticipating potential hazards ...

Contributors
Moe-Behrens, Gerd H. G., Daer, Rene, Haynes, Karmella, et al.
Created Date
2013-01-25

The rise of antibiotic resistance has emphasized the shortcomings in antibiotic drug development (Boucher et al., 2013). The move from biological based discovery methods to chemical approaches to identify candidates has left the antibiotic pipeline painfully dry (Lewis, 2013). The paucity of compounds that are effective against antibiotic resistant pathogens has led to great interest in antimicrobial peptides (AMPs) as potential solutions to the rise of resistant organisms (Hancock and Sahl, 2006; Fox, 2013). AMPs are short (5–50 amino acid) peptides that are produced by virtually all organisms as part of an innate immune system. There are 2,398 AMPs that ...

Contributors
Diehnelt, Chris, Biodesign Institute, Innovations in Medicine
Created Date
2013-12-25

Human herpesvirus 8 (HHV8) infection leads to potent activation of nuclear factor kappa B (NFκB) in primary and transformed cells. We used recombinant HHV8 (rKSHV.219) expressing green fluorescent protein under the constitutive cellular promoter elongation factor 2α and red fluorescent protein under an early HHV8 lytic gene promoter T1.1 to monitor replication during infection of human foreskin fibroblasts (HF), noting changes in NFκB activity. In primary HF, NFκB levels do not affect the ability of HHV8 to establish infection or maintain latency. Furthermore, there was no effect on the percent of cells undergoing reactivation from latency, and there were similar ...

Contributors
Blattman, Negin, Lagunoff, Michael, Blattman, Joseph, et al.
Created Date
2014-04-04

Since nitrogen (N) is often limiting in permafrost soils, we investigated the N[subscript 2]-fixing genetic potential and the inferred taxa harboring those genes by sequencing nifH gene fragments in samples taken along a permafrost thaw gradient in an Alaskan boreal soil. Samples from minimally, moderately and extensively thawed sites were taken to a depth of 79 cm to encompass zones above and below the depth of the water table. NifH reads were translated with frameshift correction and 112,476 sequences were clustered at 5% amino acid dissimilarity resulting in 1,631 OTUs. Sample depth in relation to water table depth was correlated ...

Contributors
Penton, Christopher, Yang, Caiyun, Wu, Liyou, et al.
Created Date
2016-11-24

The worst Ebola virus (EV) outbreak in history has hit Liberia, Sierra Leone and Guinea hardest and the trend lines in this crisis are grave, and now represents a global public health threat concern. Limited therapeutic and/or prophylactic options are available for people suffering from Ebola virus disease (EVD) and further complicate the situation. Previous studies suggested that the EV glycoprotein (GP) is the main determinant causing structural damage of endothelial cells that triggers the hemorrhagic diathesis, but molecular mechanisms underlying this phenomenon remains elusive. Using the informational spectrum method (ISM), a virtual spectroscopy method for analysis of the protein-protein ...

Contributors
Veljkovic, Veljko, Glisic, Sanja, Muller, Claude P., et al.
Created Date
2015-02-19

A key factor in the effectiveness of the seasonal influenza vaccine is its immunological compatibility with the circulating viruses during the season. Here we propose a new bioinformatics approach for analysis of influenza viruses which could be used as an efficient tool for selection of vaccine viruses, assessment of the effectiveness of seasonal influenza vaccines, and prediction of the epidemic/pandemic potential of novel influenza viruses.

Contributors
Veljkovic, Veljko, Paessler, Slobodan, Glisic, Sanja, et al.
Created Date
2015-12-22

The filamentous, non-heterocystous cyanobacterium Lyngbya aestuarii is an important contributor to marine intertidal microbial mats system worldwide. The recent isolate L. aestuarii BL J, is an unusually powerful hydrogen producer. Here we report a morphological, ultrastructural, and genomic characterization of this strain to set the basis for future systems studies and applications of this organism. The filaments contain circa 17 μm wide trichomes, composed of stacked disk-like short cells (2 μm long), encased in a prominent, laminated exopolysaccharide sheath. Cellular division occurs by transversal centripetal growth of cross-walls, where several rounds of division proceed simultaneously. Filament division occurs by cell ...

Contributors
Kothari, Ankita, Vaughn, Michael, Garcia-Pichel, Ferran, et al.
Created Date
2013-12-11

We examined the effect of different soil sample sizes obtained from an agricultural field, under a single cropping system uniform in soil properties and aboveground crop responses, on bacterial and fungal community structure and microbial diversity indices. DNA extracted from soil sample sizes of 0.25, 1, 5, and 10 g using MoBIO kits and from 10 and 100 g sizes using a bead-beating method (SARDI) were used as templates for high-throughput sequencing of 16S and 28S rRNA gene amplicons for bacteria and fungi, respectively, on the Illumina MiSeq and Roche 454 platforms. Sample size significantly affected overall bacterial and fungal ...

Contributors
Penton, Christopher, Gupta, Vadakattu V. S. R., Yu, Julian, et al.
Created Date
2016-06-02

With the ability to perform a multitude of unique and complex chemical transformations, microorganisms have long been the “workhorses” of many industrial processes. However, in addition to exploiting the utility of naturally evolved phenotypes, the principles, strategies, and tools of synthetic biology are now being applied to facilitate the engineering of tailor-made microbes capable of tackling some of society's most important and toughest challenges. Fueled in part by exponentially increasing reservoirs of bioinformatic data and coupled with more robust and powerful tools for its processing, research in the past decade has brought about new and broadened perspectives of fundamental biological ...

Contributors
Zhang, Weiwen, Nielsen, David, Ira A. Fulton Schools of Engineering, et al.
Created Date
2014-08-26