ASU Scholarship Showcase
This growing collection consists of scholarly works authored by ASU-affiliated faculty, students and community members, and contains many open access articles. ASU-affiliated authors are encouraged to Share Your Work in the ASU Digital Repository.
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Background Microarray image analysis processes scanned digital images of hybridized arrays to produce the input spot-level data for downstream analysis, so it can have a potentially large impact on those and subsequent analysis. Signal saturation is an optical effect that occurs when some pixel values for highly expressed genes or peptides exceed the upper detection threshold of the scanner software (2[superscript 16] - 1 = 65, 535 for 16-bit images). In practice, spots with a sizable number of saturated pixels are often flagged and discarded. Alternatively, the saturated values are used without adjustments for estimating spot intensities. The resulting expression …
- Yang, Yan, Stafford, Phillip, Kim, YoonJoo, et al.
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