ASU Electronic Theses and Dissertations
This collection includes most of the ASU Theses and Dissertations from 2011 to present. ASU Theses and Dissertations are available in downloadable PDF format; however, a small percentage of items are under embargo. Information about the dissertations/theses includes degree information, committee members, an abstract, supporting data or media.
In addition to the electronic theses found in the ASU Digital Repository, ASU Theses and Dissertations can be found in the ASU Library Catalog.
Dissertations and Theses granted by Arizona State University are archived and made available through a joint effort of the ASU Graduate College and the ASU Libraries. For more information or questions about this collection contact or visit the Digital Repository ETD Library Guide or contact the ASU Graduate College at gradformat@asu.edu.
- 1 Public
- Biophysics
- ATP synthase
- 1 Biochemistry
- 1 Biology, Plant Physiology
- 1 chloroplast
- 1 coupling ratio
- 1 recombinant expression
- more
- 1 ring stoichiometry
- 1 subunit c III ring
- Dwarf Galaxies as Laboratories of Protogalaxy Physics: Canonical Star Formation Laws at Low Metallicity
- Evolutionary Genetics of CORL Proteins
- Social Skills and Executive Functioning in Children with PCDH-19
- Deep Domain Fusion for Adaptive Image Classification
- Software Defined Pulse-Doppler Radar for Over-The-Air Applications: The Joint Radar-Communications Experiment
ATP synthase is a large multimeric protein complex responsible for generating the energy molecule adenosine triphosphate (ATP) in most organisms. The catalysis involves the rotation of a ring of c-subunits, which is driven by the transmembrane electrochemical gradient. This dissertation reports how the eukaryotic c-subunit from spinach chloroplast ATP synthase has successfully been expressed in Escherichia coli and purified in mg quantities by incorporating a unique combination of methods. Expression was accomplished using a codon optimized gene for the c-subunit, and it was expressed as an attachment to the larger, more soluble, native maltose binding protein (MBP-c1). The fusion protein …
- Contributors
- Lawrence, Robert Michael, Fromme, Petra, Chen, Julian J.L., et al.
- Created Date
- 2011