ASU Electronic Theses and Dissertations
- 2 Public
Measurements of different molecular species from single cells have the potential to reveal cell-to-cell variations, which are precluded by population-based measurements. An increasing percentage of researches have been focused on proteins, for its central roles in biological processes. Immunofluorescence (IF) has been a well-established protein analysis platform. To gain comprehensive insights into cell biology and diagnostic pathology, a crucial direction would be to increase the multiplexity of current single cell protein analysis technologies. An azide-based chemical cleavable linker has been introduced to design and synthesis novel fluorescent probes. These probes allow cyclic immunofluorescence staining which leads to the feasibility of …
- Liao, Renjie, Guo, Jia, Borges, Chad, et al.
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Quantifying molecular interactions is pivotal for understanding biological processes at molecular scale and for screening drugs. Although various detection technologies have been developed, it is still challenging to quantify the binding kinetics of small molecules because the sensitivities of the mainstream technologies scale down with the size of the molecule. To address this problem, two different optical detection methods, charge sensitive optical detection (CSOD) and virion/nano-oscillators, are developed to measure the binding-induced charge change instead of the mass change, which enables quantification of the binding kinetics for both large and small molecules. In particular, the nano-oscillator approach provides a unique …
- Ma, Guangzhong, Tao, Nongjian, Wang, Shaopeng, et al.
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