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New Developments in Isoelectric Focusing and Dielectrophoresis for Bioanalysis


Abstract Bioanalytes such as protein, cells, and viruses provide vital information but are inherently challenging to measure with selective and sensitive detection. Gradient separation technologies can provide solutions to these challenges by enabling the selective isolation and pre-concentration of bioanalytes for improved detection and monitoring. Some fundamental aspects of two of these techniques, isoelectric focusing and dielectrophoresis, are examined and novel developments are presented. A reproducible and automatable method for coupling capillary isoelectric focusing (cIEF) and matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS) based on syringe pump mobilization is found. Results show high resolution is maintained durin... (more)
Created Date 2011
Contributor Weiss, Noah (Author) / Hayes, Mark A (Advisor) / Garcia, Antonio (Committee member) / Ros, Alexandra (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Dielectrophoresis / Gradient separation / Isoelectric focusing
Type Doctoral Dissertation
Extent 167 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2011
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis