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Downstream Processing of Synechocystis for Biofuel Production


Abstract Lipids and free fatty acids (FFA) from cyanobacterium Synechocystis can be used for biofuel (e.g. biodiesel or renewable diesel) production. In order to utilize and scale up this technique, downstream processes including culturing and harvest, cell disruption, and extraction were studied. Several solvents/solvent systems were screened for lipid extraction from Synechocystis. Chloroform + methanol-based Folch and Bligh & Dyer methods were proved to be "gold standard" for small-scale analysis due to their highest lipid recoveries that were confirmed by their penetration of the cell membranes, higher polarity, and stronger interaction with hydrogen bonds. Less toxic solvents, such as methanol and MTBE, or direct transesterificati... (more)
Created Date 2011
Contributor Sheng, Jie (Author) / Rittmann, Bruce E (Advisor) / Westerhoff, Paul (Committee member) / Vermaas, Willem (Committee member) / Arizona State University (Publisher)
Subject Environmental engineering / Energy / biodiesel / bioenergy / disruption / fatty acid / lipid extraction / Synechocystis
Type Doctoral Dissertation
Extent 257 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Civil and Environmental Engineering 2011
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis