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A Low-energy, Low-cost Field Deployable Sampler For Microbial DNA Profiling

Abstract Filtration for microfluidic sample-collection devices is desirable for sample selection, concentration, preprocessing, and downstream manipulation, but microfabricating the required sub-micrometer filtration structure is an elaborate process. This thesis presents a simple method to fabricate polydimethylsiloxane (PDMS) devices with an integrated membrane filter that will sample, lyse, and extract the DNA from microorganisms in aqueous environments. An off-the-shelf membrane filter disc was embedded in a PDMS layer and sequentially bound with other PDMS channel layers. No leakage was observed during filtration. This device was validated by concentrating a large amount of cyanobacterium Synechocystis in simulated sample water with consistent ... (more)
Created Date 2011
Contributor Lecluse, Aurelie (Author) / Meldrum, Deirdre (Advisor) / Chao, Joseph (Advisor) / Meldrum, Deirdre (Advisor) / Chao, Joseph (Advisor) / Westerhoff, Paul (Committee member) / Arizona State University (Publisher)
Subject Environmental engineering / Engineering / Ocean engineering / DNA sampling / Lab on a chip / Microfluidic
Type Masters Thesis
Extent 51 pages
Language English
Reuse Permissions All Rights Reserved
Note M.S. Civil and Environmental Engineering 2011
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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