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An In Vitro Selected Sequence Capable of Ultrahigh Transgene Expression in Vaccinia Virus Infected Cells


Abstract Recombinant protein expression is essential to biotechnology and molecular medicine, but facile methods for obtaining significant quantities of folded and functional protein in mammalian cell culture have been lacking. Here I describe a novel 37-nucleotide in vitro selected sequence that promotes unusually high transgene expression in a vaccinia driven cytoplasmic expression system. Vectors carrying this sequence in a monocistronic reporter plasmid produce >1,000-fold more protein than equivalent vectors with conventional vaccinia promoters. Initial mechanistic studies indicate that high protein expression results from dual activity that impacts both transcription and translation. I suggest that this motif represents a powerful new tool ... (more)
Created Date 2012
Contributor Flores, Julia Anne (Author) / Chaput, John C (Advisor) / Jacobs, Bertram (Committee member) / Labaer, Joshua (Committee member) / Arizona State University (Publisher)
Subject Molecular biology / Biochemistry / protein expression systems / translation initiation / vaccinia virus
Type Masters Thesis
Extent 63 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note M.S. Biochemistry 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis