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Color Evolution of Kaede-type Red Fluorescent Proteins

Abstract The green fluorescent protein (GFP)-like fluorescent proteins play an important role for the color of reef-building corals. Different colors of extant coral fluorescent proteins (FPs) have evolved from a green ancestral protein. Interestingly, green-to-red photoconversion FPs (Kaede-type Red FPs) are only found in clade D from Scleractinia (Faviina suborder). Therefore, I focus on the evolution of Kaede-type FPs from Faviina suborder ancestral FP. A total of 13 mutations have been identified previously that recapitulate the evolution of Kaede-type red FPs from the ancestral green FP. To examine the effect of each mutation, total ten reconstructed FPs were analyzed and six x-ray crystal structures were solved. These substitutions created a m... (more)
Created Date 2012
Contributor Kim, Hanseong (Author) / Wachter, Rebekka M (Advisor) / Fromme, Petra (Committee member) / Redding, Kevin E (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Chemistry / Fluorescent Protein / GFP / Kaede / Photoconversion
Type Doctoral Dissertation
Extent 128 pages
Language English
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis