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The Investigation and Characterization of the Group 3 [NiFe]-Hydrogenases Using Protein Film Electrochemistry

Abstract Hydrogenases, the enzymes that reversibly convert protons and electrons to hydrogen, are used in all three domains of life. [NiFe]-hydrogenases are considered best suited for biotechnological applications because of their reversible inactivation with oxygen. Phylogenetically, there are four groups of [NiFe]-hydrogenases. The best characterized group, "uptake" hydrogenases, are membrane-bound and catalyze hydrogen oxidation in vivo. In contrast, the group 3 [NiFe]-hydrogenases are heteromultimeric, bifunctional enzymes that fulfill various cellular roles. In this dissertation, protein film electrochemistry (PFE) is used to characterize the catalytic properties of two group 3 [NiFe]-hydrogenases: HoxEFUYH from Synechocystsis sp. PCC... (more)
Created Date 2012
Contributor Mcintosh, Chelsea Lee (Author) / Jones, Anne K (Advisor) / Ghirlanda, Giovanna (Committee member) / Buttry, Daniel (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Energy / Chemistry / hydrogenase / protein electrochemistry / soluble
Type Doctoral Dissertation
Extent 196 pages
Language English
Reuse Permissions All Rights Reserved
Note Ph.D. Biochemistry 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis