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Structure Investigations of Membrane Protein OEP16


Abstract Membrane protein structure is continuing to be a topic of interest across the scientific community. However, high resolution structural data of these proteins is difficult to obtain. The amino acid transport protein, Outer Envelope Protein, 16kDa (OEP16) is a transmembrane protein channel that allows the passive diffusion of amino acids across the outer chloroplast membrane, and is used as a model protein in order to establish methods that ultimately reveal structural details about membrane proteins using nuclear magnetic resonance (NMR) spectroscopy. Methods include recombinant expression of isotope enriched inclusion bodies, purification and reconstitution in detergent micelles, and pre-characterization techniques including circular dichr... (more)
Created Date 2012
Contributor Zook, James Duncan (Author) / Fromme, Petra (Advisor) / Chen, Julian (Committee member) / Wachter, Rebekka (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Membrane Protein / OEP16
Type Doctoral Dissertation
Extent 167 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Biochemistry 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis