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A Chip for Hydrodynamic Microvortical Rotation of Live Single Cells


Abstract Single cell analysis has become increasingly important in understanding disease onset, progression, treatment and prognosis, especially when applied to cancer where cellular responses are highly heterogeneous. Through the advent of single cell computerized tomography (Cell-CT), researchers and clinicians now have the ability to obtain high resolution three-dimensional (3D) reconstructions of single cells. Yet to date, no live-cell compatible version of the technology exists. In this thesis, a microfluidic chip with the ability to rotate live single cells in hydrodynamic microvortices about an axis parallel to the optical focal plane has been demonstrated. The chip utilizes a novel 3D microchamber design arranged beneath a main channel creat... (more)
Created Date 2012
Contributor Myers, Jakrey (Author) / Meldrum, Deirdre (Advisor) / Johnson, Roger (Committee member) / Frakes, David (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Biology / Biophysics / 3D / Diffusive Lithography / Live Cell / Microfluidics / Microvortex / Tomography
Type Masters Thesis
Extent 104 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note M.S. Bioengineering 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis