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AFM study of gene silencing by DNA methylation and its interactions involving chromatin and methyl CpG binding proteins


Abstract CpG methylation is an essential requirement for the normal development of mammals, but aberrant changes in the methylation can lead to tumor progression and cancer. An in-depth understanding of this phenomenon can provide insights into the mechanism of gene repression. We present a study comparing methylated DNA and normal DNA wrt its persistence length and contour length. Although, previous experiments and studies show no difference between the physical properties of the two, the data collected and interpreted here gives a different picture to the methylation phenomena and its effect on gene silencing. The study was extended to the artificially reconstituted chromatin and its interactions with the methyl CpG binding proteins were also prob... (more)
Created Date 2012
Contributor Kaur, Parminder (Author) / Lindsay, Stuart (Advisor) / Ros, Robert (Committee member) / Tao, Nongjian (Committee member) / Vaiana, Sara (Committee member) / Beckenstein, Oliver (Committee member) / Arizona State University (Publisher)
Subject Biophysics / Physics / Oncology
Type Doctoral Dissertation
Extent 175 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Physics 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis