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Design of Apoferritin-Based Nanoparticle MRI Contrast Agents Through Controlled Metal Deposition


Abstract Sensitivity is a fundamental challenge for in vivo molecular magnetic resonance imaging (MRI). Here, I improve the sensitivity of metal nanoparticle contrast agents by strategically incorporating pure and doped metal oxides in the nanoparticle core, forming a soluble, monodisperse, contrast agent with adjustable T2 or T1 relaxivity (r2 or r1). I first developed a simplified technique to incorporate iron oxides in apoferritin to form "magnetoferritin" for nM-level detection with T2- and T2* weighting. I then explored whether the crystal could be chemically modified to form a particle with high r1. I first adsorbed Mn2+ ions to metal binding sites in the apoferritin pores. The strategic placement of metal ions near sites of water ex... (more)
Created Date 2012
Contributor Clavijo Jordan, Maria Veronica (Author) / Bennett, Kevin M (Advisor) / Kodibagkar, Vikram (Committee member) / Sherry, A Dean (Committee member) / Wang, Xiao (Committee member) / Yarger, Jeffery (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Biophysics / Apoferritin / Manganese / MRI / Tungsten
Type Doctoral Dissertation
Extent 144 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Bioengineering 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis