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Microscale Electroporation for Transfection of Genetic Constructs into Adherent Secondary Cells and Primary Neurons in Culture


Abstract Gene manipulation techniques, such as RNA interference (RNAi), offer a powerful method for elucidating gene function and discovery of novel therapeutic targets in a high-throughput fashion. In addition, RNAi is rapidly being adopted for treatment of neurological disorders, such as Alzheimer's disease (AD), Parkinson's disease, etc. However, a major challenge in both of the aforementioned applications is the efficient delivery of siRNA molecules, plasmids or transcription factors to primary cells such as neurons. A majority of the current non-viral techniques, including chemical transfection, bulk electroporation and sonoporation fail to deliver with adequate efficiencies and the required spatial and temporal control. In this study, ... (more)
Created Date 2012
Contributor Patel, Chetan (Author) / Muthuswamy, Jitendran (Advisor) / Helms Tillery, Stephen (Committee member) / Jain, Tilak (Committee member) / Caplan, Michael (Committee member) / Vernon, Brent (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Electroporation / Microelectrode array / neuron / siRNA delivery / transfection
Type Doctoral Dissertation
Extent 187 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Bioengineering 2012
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis