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Studying the Solution Behavior of DNA and DNA Sliding Clamps Using Various Fluorescence Techniques


Abstract Solution conformations and dynamics of proteins and protein-DNA complexes are often difficult to predict from their crystal structures. The crystal structure only shows a snapshot of the different conformations these biological molecules can have in solution. Multiple different conformations can exist in solution and potentially have more importance in the biological activity. DNA sliding clamps are a family of proteins with known crystal structures. These clamps encircle the DNA and enable other proteins to interact more efficiently with the DNA. Eukaryotic PCNA and prokaryotic β clamp are two of these clamps, some of the most stable homo-oligomers known. However, their solution stability and conformational equilibrium have not be... (more)
Created Date 2013
Contributor Ranjit, Suman (Author) / Levitus, Marcia (Advisor) / Lindsay, Stuart (Committee member) / Yan, Hao (Committee member) / Arizona State University (Publisher)
Subject Biophysics / Physical chemistry / DNA / FCS / Fluorescence / FRET / Sliding clamp / smFluorescence
Type Doctoral Dissertation
Extent 224 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis