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From Autopsy Donor to Stem Cell to Neuron (and Back Again): Cell Line Cohorts, IPSC Proof-of-Principle Studies, and Transcriptome Comparisons of In Vitro and In Vivo Neural Cells


Abstract Induced pluripotent stem cells (iPSCs) are an intriguing approach for neurological disease modeling, because neural lineage-specific cell types that retain the donors' complex genetics can be established in vitro. The statistical power of these iPSC-based models, however, is dependent on accurate diagnoses of the somatic cell donors; unfortunately, many neurodegenerative diseases are commonly misdiagnosed in live human subjects. Postmortem histopathological examination of a donor's brain, combined with premortem clinical criteria, is often the most robust approach to correctly classify an individual as a disease-specific case or unaffected control. We describe the establishment of primary dermal fibroblasts cells lines from 28 autop... (more)
Created Date 2013
Contributor Hjelm, Brooke Erika (Author) / Craig, David W. (Advisor) / Wilson-Rawls, Norma J. (Advisor) / Huentelman, Matthew J. (Committee member) / Mason, Hugh S. (Committee member) / Kusumi, Kenro (Committee member) / Arizona State University (Publisher)
Subject Molecular biology / Genetics / Neurosciences / autopsy / genomics / induced pluripotent stem cells / neural differentiation / RNA-Seq / stem cell biology
Type Doctoral Dissertation
Extent 291 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Molecular and Cellular Biology 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis