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Study of Ribosomes having Modifications in the Peptidyltransferase Center Using Non-α-L-Amino Acids and Synthesis and Biological Evaluation of Topopyrones


Abstract The ribosome is a ribozyme and central to the biosynthesis of proteins in all organisms. It has a strong bias against non-alpha-L-amino acids, such as alpha-D-amino acids and beta-amino acids. Additionally, the ribosome is only able to incorporate one amino acid in response to one codon. It has been demonstrated that reengineering of the peptidyltransferase center (PTC) of the ribosome enabled the incorporation of both alpha-D-amino acids and beta-amino acids into full length protein. Described in Chapter 2 are five modified ribosomes having modifications in the peptidyltrasnferase center in the 23S rRNA. These modified ribosomes successfully incorporated five different beta-amino acids (2.1 - 2.5) into E. coli dihydrofolate reductase (DHFR... (more)
Created Date 2013
Contributor Maini, Rumit (Author) / Hecht, Sidney M (Advisor) / Gould, Ian (Committee member) / Yan, Hao (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Biochemistry / in vitro protein expression / modified ribosomes / peptidomimetics / ribosomes / topopyrones
Type Doctoral Dissertation
Extent 144 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis