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Optimization and Parametric Characterization of a Hydrodynamic Microvortex Chip for Single Cell Rotation

Abstract Volumetric cell imaging using 3D optical Computed Tomography (cell CT) is advantageous for identification and characterization of cancer cells. Many diseases arise from genomic changes, some of which are manifest at the cellular level in cytostructural and protein expression (functional) features which can be resolved, captured and quantified in 3D far more sensitively and specifically than in traditional 2D microscopy. Live single cells were rotated about an axis perpendicular to the optical axis to facilitate data acquisition for functional live cell CT imaging. The goal of this thesis research was to optimize and characterize the microvortex rotation chip. Initial efforts concentrated on optimizing the microfabrication process in terms o... (more)
Created Date 2013
Contributor Shetty, Rishabh M. (Author) / Meldrum, Deirdre R (Advisor) / Johnson, Roger H (Committee member) / Tillery, Stephen H (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Cell rotation / Microfabrication / Microvortex / Optical tweezers / Optimization
Type Masters Thesis
Extent 78 pages
Language English
Reuse Permissions All Rights Reserved
Note M.S. Bioengineering 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis
13.3 MB video/x-ms-asf
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Description Demonstration of process flow in the microvortex chip
54.2 MB video/x-ms-asf
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Description Cell rotation in a 50 microns wide (at the neck) trapezoidal chamber,at a flow rate of 95 microliters/min at approximately 0.25 rev/s
12.3 MB video/x-ms-asf
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Description Cell rotation in a 70 microns wide (at the neck) trapezoidal chamber,at a flow rate of 7 microliters/min at approximately 0.125 rev/s