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Targeting Astrogliosis: Isolation and Characterization of Astrocyte Specific Single Chain Antibody Fragments


Abstract Specificity and affinity towards a given ligand/epitope limit target-specific delivery. Companies can spend between $500 million to $2 billion attempting to discover a new drug or therapy; a significant portion of this expense funds high-throughput screening to find the most successful target-specific compound available. A more recent addition to discovering highly specific targets is the application of phage display utilizing single chain variable fragment antibodies (scFv). The aim of this research was to employ phage display to identify pathologies related to traumatic brain injury (TBI), particularly astrogliosis. A unique biopanning method against viable astrocyte cultures activated with TGF-β achieved this aim. Four scFv clon... (more)
Created Date 2013
Contributor Marsh, William (Author) / Stabenfeldt, Sarah (Advisor) / Caplan, Michael (Committee member) / Sierks, Michael (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Biochemistry / astrocyte / astrogliosis / phage / scfv / tbi
Type Masters Thesis
Extent 49 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note M.S. Bioengineering 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis