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Novel Strategies for Producing Proteins with Non-Proteinogenic Amino Acids


Abstract The biological and chemical diversity of protein structure and function can be greatly expanded by position-specific incorporation of non-natural amino acids bearing a variety of functional groups. Non-cognate amino acids can be incorporated into proteins at specific sites by using orthogonal aminoacyl-tRNA synthetase/tRNA pairs in conjunction with nonsense, rare, or 4-bp codons. There has been considerable progress in developing new types of amino acids, in identifying novel methods of tRNA aminoacylation, and in expanding the genetic code to direct their position. Chemical aminoacylation of tRNAs is accomplished by acylation and ligation of a dinucleotide (pdCpA) to the 3'-terminus of truncated tRNA. This strategy allows the incorpora... (more)
Created Date 2013
Contributor Nangreave, Ryan Christopher (Author) / Hecht, Sidney M (Advisor) / Yan, Hao (Committee member) / Gould, Ian (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Biochemistry / Amino Acid / Aminoacylated / Non-proteinogenic / Protein / Suppressor tRNA
Type Doctoral Dissertation
Extent 146 pages
Language English
Rights All Rights Reserved
Note Ph.D. Chemistry 2013
Collaborating Institutions ASU Graduate College / ASU Libraries
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis