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Small Molecule Detection by Surface Plasmon Resonance: Improvements in Sensitivity and Kinetic Measurement

Abstract Surface plasmon resonance (SPR) has emerged as a popular technique for elucidating subtle signals from biological events in a label-free, high throughput environment. The efficacy of conventional SPR sensors, whose signals are mass-sensitive, diminishes rapidly with the size of the observed target molecules. The following work advances the current SPR sensor paradigm for the purpose of small molecule detection. The detection limits of two orthogonal components of SPR measurement are targeted: speed and sensitivity. In the context of this report, speed refers to the dynamic range of measured kinetic rate constants, while sensitivity refers to the target molecule mass limitation of conventional SPR measurement. A simple device for high-speed ... (more)
Created Date 2013
Contributor Macgriff, Christopher Assiff (Author) / Tao, Nongjian (Advisor) / Wang, Shaopeng (Committee member) / Labaer, Joshua (Committee member) / Chae, Junseok (Committee member) / Arizona State University (Publisher)
Subject Engineering / Electrical engineering / Biophysics / Impedance / Kinetics / Microfluidics / Microscopy / Small molecule / Surface Plasmon Resonance
Type Doctoral Dissertation
Extent 124 pages
Language English
Reuse Permissions All Rights Reserved
Note Ph.D. Electrical Engineering 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis