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Single cell RT-qPCR based ocean environmental sensing device development


Abstract This thesis research focuses on developing a single-cell gene expression analysis method for marine diatom Thalassiosira pseudonana and constructing a chip level tool to realize the single cell RT-qPCR analysis. This chip will serve as a conceptual foundation for future deployable ocean monitoring systems. T. pseudonana, which is a common surface water microorganism, was detected in the deep ocean as confirmed by phylogenetic and microbial community functional studies. Six-fold copy number differences between 23S rRNA and 23S rDNA were observed by RT-qPCR, demonstrating the moderate functional activity of detected photosynthetic microbes in the deep ocean including T. pseudonana. Because of the ubiquity of T. pseudonana, it is a good candid... (more)
Created Date 2013
Contributor Shi, Xu (Author) / Meldrum, Deirdre R. (Advisor) / Zhang, Weiwen (Committee member) / Chao, Shih-Hui (Committee member) / Westerhoff, Paul (Committee member) / Arizona State University (Publisher)
Subject Environmental engineering / Biological oceanography / Microbiology / environmental monitoring / gene expression / miniature device / single cell
Type Doctoral Dissertation
Extent 159 pages
Language English
Copyright
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Note Ph.D. Civil and Environmental Engineering 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis