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Insulator Based Dielectrophoretic Trapping of Single Mammalian Cells


Abstract This work demonstrated a novel microfluidic device based on direct current (DC) insulator based dielectrophoresis (iDEP) for trapping individual mammalian cells in a microfluidic device. The novel device is also applicable for selective trapping of weakly metastatic mammalian breast cancer cells (MCF-7) from mixtures with mammalian Peripheral Blood Mononuclear Cells (PBMC) and highly metastatic mammalian breast cancer cells, MDA-MB-231. The advantage of this approach is the ease of integration of iDEP structures in microfliudic channels using soft lithography, the use of DC electric fields, the addressability of the single cell traps for downstream analysis and the straightforward multiplexing for single cell trapping. These microfluidic de... (more)
Created Date 2013
Contributor Bhattacharya, Sanchari (Author) / Ros, Alexandra (Advisor) / Ros, Alexandra (Committee member) / Ros, Robert (Committee member) / Buttry, Daniel (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Cancer cells / Dielectrophoresis / MCF-7 / Microfluidics / Separation / Single cells
Type Doctoral Dissertation
Extent 90 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Ph.D. Chemistry 2013
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis