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The Investigation and Characterization of Redox Enzymes Using Protein Film Electrochemistry


Abstract Redox reactions are crucial to energy transduction in biology. Protein film electrochemistry (PFE) is a technique for studying redox proteins in which the protein is immobilized at an electrode surface so as to allow direct exchange of electrons. Establishing a direct electronic connection eliminates the need for redox­active mediators, thus allowing for interrogation of the redox protein of interest. PFE has proven a versatile tool that has been used to elucidate the properties of many technologically relevant redox proteins including hydrogenases, laccases, and glucose oxidase.

This dissertation is comprised of two parts: extension of PFE to a novel electrode material and application of PFE to the investigation of a new type of hydroge... (more)
Created Date 2014
Contributor Kwan, Patrick (Author) / Jones, Anne K (Advisor) / Francisco, Wilson (Committee member) / Moore, Thomas (Committee member) / Arizona State University (Publisher)
Subject Biochemistry / Chemistry / Inorganic chemistry / Antimony Tin Oxide / Catalytic Bias / Hydrogenase / Oxygen Tolerance / Protein Film Electrochemistry / Pyrococcus furiosus
Type Doctoral Dissertation
Extent 137 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Biochemistry 2014
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis