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3 ' LIFE: a functional assay to detect miRNA targets in high-throughput


Abstract MicroRNAs (miRNAs) are short non-coding RNAs that regulate gene output at the post-transcriptional level by targeting degenerate elements primarily in 3′untranslated regions (3′UTRs) of mRNAs. Individual miRNAs can regulate networks of hundreds of genes, yet for the majority of miRNAs few, if any, targets are known. Misexpression of miRNAs is also a major contributor to cancer progression, thus there is a critical need to validate miRNA targets in high-throughput to understand miRNAs' contribution to tumorigenesis. Here we introduce a novel high-throughput assay to detect miRNA targets in 3′UTRs, called Luminescent Identification of Functional Elements in 3′UTRs (3′LIFE). We demonstrate the feasibility of 3′LIFE using a data set of 275 ... (more)
Created Date 2014-09-29
Contributor Wolter, Justin (ASU author) / Kotagama, Kasuen (ASU author) / Pierre-Bez, Alexandra C. (ASU author) / Firago, Mari (ASU author) / Mangone, Marco (ASU author) / College of Liberal Arts and Sciences / School of Life Sciences / Biodesign Institute / Personalized Diagnostics / Barrett, the Honors College
Series NUCLEIC ACIDS RESEARCH
Type Text
Extent 17 pages
Language English
Identifier DOI: 10.1093/nar/gku626 / ISSN: 1362-4962 / ISSN: 0305-1048
Copyright
Reuse Permissions All Rights Reserved
Citation Wolter, Justin M., Kotagama, Kasuen, Pierre-Bez, Alexandra C., Firago, Mari, & Mangone, Marco (2014). 3 ' LIFE: a functional assay to detect miRNA targets in high-throughput. NUCLEIC ACIDS RESEARCH, 42(17), 0-0. http://nar.oxfordjournals.org.ezproxy1.lib.asu.edu/content/early/2014/07/29/nar.gku626.short
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


  Nucl Acids Res 2014- Wolter nar gku 626
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