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Investigating Dynamics Using Three Systems: Cy3 on DNA, ME1 Heterodimers, and DNA Processivity Clamps


Abstract Biophysical techniques have been increasingly applied toward answering biological questions with more precision. Here, three different biological systems were studied with the goal of understanding their dynamic differences, either conformational dynamics within the system or oligomerization dynamics between monomers. With Cy3 on the 5' end of DNA, the effects of changing the terminal base pair were explored using temperature-dependent quantum yields. It was discovered, in combination with simulations, that a terminal thymine base has the weakest stacking interactions with the Cy3 dye compared to the other three bases. With ME1 heterodimers, the goal was to see if engineering a salt bridge at the dimerization interface could allow f... (more)
Created Date 2015
Contributor Binder, Jennifer K. (Author) / Levitus, Marcia (Advisor) / Wachter, Rebekka (Committee member) / Ros, Robert (Committee member) / Arizona State University (Publisher)
Subject Chemistry / Biochemistry / Biophysics / beta clamp / DNA / fluorescence correlation spectroscopy / PCNA / processivity clamps / sliding clamps
Type Doctoral Dissertation
Extent 135 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Chemistry 2015
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis