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Quantifying Mechanical Heterogeneity in 3D Biological Systems with the Atomic Force Microscope

Abstract The atomic force microscope (AFM) is capable of directly probing the mechanics of samples with length scales from single molecules to tissues and force scales from pico to micronewtons. In particular, AFM is widely used as a tool to measure the elastic modulus of soft biological samples by collecting force-indentation relationships and fitting these to classic elastic contact models. However, the analysis of raw force-indentation data may be complicated by mechanical heterogeneity present in biological systems. An analytical model of an elastic indentation on a bonded two-layer sample was solved. This may be used to account for substrate effects and more generally address experimental design for samples with varying elasticity. This model w... (more)
Created Date 2015
Contributor Doss, Bryant Lee (Author) / Ros, Robert (Advisor) / Lindsay, Stuart (Committee member) / Nikkhah, Mehdi (Committee member) / Beckstein, Oliver (Committee member) / Arizona State University (Publisher)
Subject Biophysics / Physics / Biomechanics / Atomic force microscopy / Cell mechanics / Mechanobiology / Metastasis / Microrheometry
Type Doctoral Dissertation
Extent 166 pages
Language English
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Physics 2015
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis