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Immunogenic Subviral Particles Displaying Domain III of Dengue 2 Envelope Protein Vectored by Measles Virus


Abstract Vaccines against the arthropod-borne dengue virus (DENV) are still commercially nonexistent. A subunit immunization strategy may be of value, especially if a safe viral vector acts as a biologically active adjuvant. The DENV envelope protein (E), the main target for neutralizing immune responses, has three conformational domains. The immunoglobulin-like and independently folding domain III (DIII) contains epitopes that elicit highly specific neutralizing antibodies. The hepatitis B small surface antigen (HBsAg, S) was used as a scaffold to display DENV 2 DIII on a virus-like particle (VLP). A measles virus (MV) was engineered to vector HBsAg and the hybrid glycoprotein DIII-HBsAg in two different loci (DIII-S). Despite the relatively delete... (more)
Created Date 2015
Contributor Harahap, Indira Saridewi (Author) / Reyes del Valle, Jorge (Advisor) / Hogue, Brenda G (Advisor) / Lake, Douglas (Committee member) / Mason, Hugh (Committee member) / Arizona State University (Publisher)
Subject Virology / Molecular biology / Microbiology / Dengue / Flavivirus / HBsAg / Measles / Recombinant vaccine / VLP
Type Masters Thesis
Extent 97 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Masters Thesis Biology 2015
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis