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Optimization and Ultimate Limitations for Immunoassay and Clinical Diagnostics

Abstract Biological fluids, in particular blood plasma, provide a vital source of information on the state of human health. While specific detection of biomarker species can aid in disease diagnostics, the complexity of plasma makes analysis challenging. Despite the challenge of complex sample analysis, biomarker quantification has become a primary interest in biomedical analysis. Due to the extremely specific interaction between antibody and analyte, immunoassays are attractive for the analysis of these samples and have gained popularity since their initial introduction several decades ago. Current limitations to diagnostics through blood testing include long incubation times, interference from non-specific binding, and the requirement for speciali... (more)
Created Date 2015
Contributor Woolley, Christine F (Author) / Hayes, Mark A (Advisor) / Ros, Alexandra (Committee member) / LaBaer, Joshua (Committee member) / Arizona State University (Publisher)
Subject Analytical chemistry / Biochemistry / assay optimization / microbead immunoassay / microscale separation / quantitation limit / sandwich immunoassay
Type Doctoral Dissertation
Extent 255 pages
Language English
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Chemistry 2015
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS

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Description Dissertation/Thesis