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Biosensor Platform for Rapid Detection of E. coli in Drinking Water


Abstract The need for rapid, specific and sensitive assays that provide a detection of bacterial indicators are important for monitoring water quality. Rapid detection using biosensor is a novel approach for microbiological testing applications. Besides, validation of rapid methods is an obstacle in adoption of such new bio-sensing technologies. In this study, the strategy developed is based on using the compound 4-methylumbelliferyl glucuronide (MUG), which is hydrolyzed rapidly by the action of E. coli β-D-glucuronidase (GUD) enzyme to yield a fluorogenic product that can be quantified and directly related to the number of E. coli cells present in water samples. The detection time required for the biosensor response ranged from 30 to 120 minut... (more)
Created Date 2015
Contributor Hesari, Nikou (Author) / Abbaszadegan, Morteza (Advisor) / Alum, Absar (Committee member) / Fox, Peter (Committee member) / Stout, Valerie (Committee member) / Arizona State University (Publisher)
Subject Civil engineering / Environmental engineering / 4-methylumbelliferyl glucuronide / E. coli / Rapid detection / water
Type Doctoral Dissertation
Extent 78 pages
Language English
Copyright
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Note Doctoral Dissertation Civil and Environmental Engineering 2015
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis