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Analysis of Acyl Carrier Protein in the cyanobacterium Synechocystis sp. PCC 6803


Abstract Acyl Carrier Protein (ACP) is a small, acidic protein that plays an essential role in fatty acid synthesis by elongating fatty acid chains. ACP was isolated from an extract of a modified strain of Synechocystis sp. PCC 6803 that contains a thioesterase and from which the acyl-ACP synthetase has been deleted. Using ammonium sulfate precipitation to isolate a crude protein fraction containing ACP, immunoblot analysis was performed to determine relative amounts of free and acylated-ACP in the cell. The nature of fatty acids attached to ACP was determined by creating butylamide derivatives that were analyzed using GC/MS. Immunoblot analysis showed a roughly 1:1 ratio of acylated ACP to free ACP in the cell depending on the nutritional state of ... (more)
Created Date 2016-05
Contributor Wu, Sharon Gao (Author) / Vermaas, Willem (Thesis Director) / Redding, Kevin (Committee Member) / School of Sustainability / School of Mathematical and Statistical Sciences / School of Molecular Sciences / School of International Letters and Cultures / School of Life Sciences / Barrett, The Honors College
Subject Acyl Carrier Protein / Biofuels / Fatty Acid Synthesis / Cyanobacteria
Series Academic Year 2015-2016
Type Text
Extent 54 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Collaborating Institutions Barrett, the Honors College
Additional Formats MODS / OAI Dublin Core / RIS


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