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Characterization of Functionally Relevant Resides of Escherichia coli Multi-Drug Efflux Pump Protein AcrB


Abstract Emergence of multidrug resistant (MDR) bacteria is a major concern to global health. One of the major MDR mechanisms bacteria employ is efflux pumps for the expulsion of drugs from the cell. In Escherichia coli, AcrAB-TolC proteins constitute the major chromosomally-encoded drug efflux system. AcrB, a trimeric membrane protein is well-known for its substrate promiscuity. It has the ability to efflux a broad spectrum of substrates alongside compounds such as dyes, detergent, bile salts and metabolites. Newly identified AcrB residues were shown to be functionally relevant in the drug binding and translocation pathway using a positive genetic selection strategy. These residues—Y49, V127, D153, G288, F453, and L486—were identified as the sites ... (more)
Created Date 2016
Contributor Blake, Mellecha Rose (Author) / Misra, Rajeev (Advisor) / Stout, Valerie (Committee member) / Wang, Xuan (Committee member) / Arizona State University (Publisher)
Subject Biology / Microbiology / AcrAB-TolC / AcrB / binding and translocation pathway / efflux pump / F610A
Type Masters Thesis
Extent 69 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Masters Thesis Biology 2016
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis