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Single Cell RT-qPCR on 3D Cell Spheroids


Abstract A single cell is the very fundamental element in an organism; however, it contains the most complicated and stochastic information, such as DNA, RNA, and protein expression. Thus, it is a necessity to study stochastic gene expression in order to discover the biosignatures at the single-cell level. The heterogeneous gene expression of single cells from an isogenic cell population has already been studied for years. Yet to date, single-cell studies have been confined in a fashion of analyzing isolated single cells or a dilution of cells from the bulk-cell populations. These techniques or devices are limited by either the mechanism of cell lysis or the difficulties to target specific cells without harming neighboring cells.

This dissertation ... (more)
Created Date 2016
Contributor Wang, Kuo-Chen (Author) / Meldrum, Deirdre R (Advisor) / Chao, Shih-hui (Committee member) / Wang, Hong (Committee member) / Goryll, Michael (Committee member) / Arizona State University (Publisher)
Subject Electrical engineering / Biomedical engineering
Type Doctoral Dissertation
Extent 108 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Electrical Engineering 2016
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis