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5′ and 3′ Untranslated Regions Strongly Enhance Performance of Geminiviral Replicons in Nicotiana benthamiana Leaves


Abstract We previously reported a recombinant protein production system based on a geminivirus replicon that yields high levels of vaccine antigens and monoclonal antibodies in plants. The bean yellow dwarf virus (BeYDV) replicon generates massive amounts of DNA copies, which engage the plant transcription machinery. However, we noticed a disparity between transcript level and protein production, suggesting that mRNAs could be more efficiently utilized. In this study, we systematically evaluated genetic elements from human, viral, and plant sources for their potential to improve the BeYDV system. The tobacco extensin terminator enhanced transcript accumulation and protein production compared to other commonly used terminators, indicating that effici... (more)
Created Date 2016-02-24
Contributor Diamos, Andy (ASU author) / Rosenthal, Sun (ASU author) / Mason, Hugh (ASU author) / Biodesign Institute / Immunotherapy, Vaccines and Virotherapy
Series FRONTIERS IN PLANT SCIENCE
Type Text
Extent 15 pages
Language English
Identifier DOI: 10.3389/fpls.2016.00200 / ISSN: 1664-462X
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Citation Diamos, A. G., Rosenthal, S. H., & Mason, H. S. (2016). 5′ and 3′ Untranslated Regions Strongly Enhance Performance of Geminiviral Replicons in Nicotiana benthamiana Leaves. Frontiers in Plant Science, 7. doi:10.3389/fpls.2016.00200
Note View the article as published at http://journal.frontiersin.org/article/10.3389/fpls.2016.00200/full
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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