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The Effect of GATA6 Expression and Its Neighborhood Impact Factor on Regulating Cell Fate


Abstract A genetically engineered line of human induced pluripotent stem cells was used to study the effects of gene expression on cell fate. These cells were designed to activate expression of the gene GATA6 when exposed to the small molecule doxycycline. This gene was chosen because it plays an important role in the developmental biology stages of liver formation. Because of the way the cells were engineered, a given population would have a heterogeneous expression of GATA6 because each cell could have a different copy number of the exogenous gene. This variation allows for the differentiation of multiple cell types, and is used to grow liver organoids. The early liver organoid samples were studied via immunofluorescent staining, imaging, and... (more)
Created Date 2017
Contributor Carter, Shaylina Rae (Author) / Ebrahimkhani, Mohammad R (Advisor) / Kiani, Samira (Advisor) / Nikkhah, Mehdi (Committee member) / Arizona State University (Publisher)
Subject Biomedical engineering / Developmental biology / communication / gata6 / neighborhood / organoid
Type Masters Thesis
Extent 111 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Masters Thesis Bioengineering 2017
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis
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Description Powerpoint presentation used in the defense.