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Investigation of DNA Methylation in Obesity and its Underlying Insulin Resistance


Abstract Obesity and its underlying insulin resistance are caused by environmental and genetic factors. DNA methylation provides a mechanism by which environmental factors can regulate transcriptional activity. The overall goal of the work herein was to (1) identify alterations in DNA methylation in human skeletal muscle with obesity and its underlying insulin resistance, (2) to determine if these changes in methylation can be altered through weight-loss induced by bariatric surgery, and (3) to identify DNA methylation biomarkers in whole blood that can be used as a surrogate for skeletal muscle.

Assessment of DNA methylation was performed on human skeletal muscle and blood using reduced representation bisulfite sequencing (RRBS) for high-thr... (more)
Created Date 2017
Contributor Day, Samantha Elaine (Author) / Coletta, Dawn K. (Advisor) / Katsanos, Christos (Committee member) / Mandarino, Lawrence J. (Committee member) / Shaibi, Gabriel Q. (Committee member) / Dinu, Valentin (Committee member) / Arizona State University (Publisher)
Subject Biology / Genetics / Endocrinology / DNA methylation / Epigenetics / Insulin resistance / Next generation sequencing / Obesity / Skeletal muscle
Type Doctoral Dissertation
Extent 139 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Biology 2017
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis
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Description Appendix A
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Description Appendix B
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Description Appendix C
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Description Appendix D
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Description Appendix G