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An Integrated Microarray Printing and Detection System to Study Protein-Protein Interactions


Abstract In this thesis, a breadboard Integrated Microarray Printing and Detection System (IMPDS) was proposed to address key limitations of traditional microarrays. IMPDS integrated two core components of a high-resolution surface plasmon resonance imaging (SPRi) system and a piezoelectric dispensing system that can print ultra-low volume droplets. To avoid evaporation of droplets in the microarray, a 100 μm thick oil layer (dodecane) was used to cover the chip surface. The interaction between BSA (Bovine serum albumin) and Anti-BSA was used to evaluate the capability of IMPDS. The alignment variability of printing, stability of droplets array and quantification of protein-protein interactions based on nanodroplet array were evaluated through a 10 ... (more)
Created Date 2017
Contributor Xiao, Feng (Author) / Tao, Nongjian (Advisor) / Borges, Chad (Committee member) / Guo, Jia (Committee member) / Arizona State University (Publisher)
Subject Chemistry
Type Masters Thesis
Extent 40 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Masters Thesis Chemistry 2017
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis