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Approaches to Engineering Synechocystis for Biofuel Production with Emphasis on Electron Transport Modifications


Abstract The basic scheme for photosynthesis suggests the two photosystems existing in parity with one another. However, cyanobacteria typically maintain significantly more photosystem I (PSI) than photosystem II (PSII) complexes. I set out to evaluate this disparity through development and analysis of multiple mutants of the genetically tractable cyanobacterium Synechocystis sp. PCC 6803 that exhibit a range of expression levels of the main proteins present in PSI (Chapter 2). One hypothesis was that the higher abundance of PSI in this organism is used to enable more cyclic electron flow (CEF) around PSI to contribute to greater ATP synthesis. Results of this study show that indeed CEF is enhanced by the high amount of PSI present in WT. On the oth... (more)
Created Date 2017
Contributor Moore, Vicki (Author) / Vermaas, Willem (Advisor) / Wang, Xuan (Committee member) / Roberson, Robert (Committee member) / Gaxiola, Roberto (Committee member) / Bingham, Scott (Committee member) / Arizona State University (Publisher)
Subject Molecular biology / Microbiology / Biochemistry / bioenergetics / biofuel / bioreactor / cyanobacteria / metabolic engineering / photosynthesis
Type Doctoral Dissertation
Extent 156 pages
Language English
Copyright
Reuse Permissions All Rights Reserved
Note Doctoral Dissertation Biology 2017
Collaborating Institutions Graduate College / ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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Description Dissertation/Thesis