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Regulation of cancer epigenomes with a histone-binding synthetic transcription factor


Abstract Chromatin proteins have expanded the mammalian synthetic biology toolbox by enabling control of active and silenced states at endogenous genes. Others have reported synthetic proteins that bind DNA and regulate genes by altering chromatin marks, such as histone modifications. Previously, we reported the first synthetic transcriptional activator, the “Polycomb-based transcription factor” (PcTF) that reads histone modifications through a protein–protein interaction between the polycomb chromodomain motif and trimethylated lysine 27 of histone H3 (H3K27me3). Here, we describe the genome-wide behavior of the polycomb-based transcription factor fusion protein. Transcriptome and chromatin profiling revealed several polycomb-based transcription fa... (more)
Created Date 2017-01-09
Contributor Nyer, David (ASU author) / Daer, Rene (ASU author) / Vargas, Daniel (ASU author) / Hom, Caroline (ASU author) / Haynes, Karmella (ASU author) / Ira A. Fulton Schools of Engineering / School of Biomedical and Health Systems Engineering
Series NPJ GENOMIC MEDICINE
Type Text
Extent 10 pages
Language English
Identifier DOI: 10.1038/s41525-016-0002-3 / ISSN: 2056-7944
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Citation Nyer, D. B., Daer, R. M., Vargas, D., Hom, C., & Haynes, K. A. (2017). Regulation of cancer epigenomes with a histone-binding synthetic transcription factor. Npj Genomic Medicine, 2(1). doi:10.1038/s41525-016-0002-3
Note The final version of this article, as published in Npj Genomic Medicine, can be viewed online at: http://www.nature.com/articles/s41525-016-0002-3
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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