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Functional Characterization of Two Elongases of Very Long-Chain Fatty Acid from Tenebrio molitor L. (Coleoptera: Tenebrionidae)


Abstract The elongases of very long chain fatty acid (ELOVL or ELO) are essential in the biosynthesis of fatty acids longer than C14. Here, two ELO full-length cDNAs (TmELO1, TmELO2) from the yellow mealworm (Tenebrio molitor L.) were isolated and the functions were characterized. The open reading frame (ORF) lengths of TmELO1 and TmELO2 were 1005 bp and 972 bp, respectively and the corresponding peptide sequences each contained several conserved motifs including the histidine-box motif HXXHH. Phylogenetic analysis demonstrated high similarity with the ELO of Tribolium castaneum and Drosophila melanogaster. Both TmELO genes were expressed at various levels in eggs, 1st and 2nd instar larvae, mature larvae, pupae, male and female adults. Injection of... (more)
Created Date 2017-09-08
Contributor Zheng, Tianxiang (Author) / Li, Hongshuang (Author) / Han, Na (Author) / Wang, Shengyin (Author) / Hackney Price, Jennifer (ASU author) / Wang, Minzi (Author) / Zhang, Dayu (Author) / Arizona State University. New College of Interdisciplinary Arts and Sciences. School of Mathematical and Natural Sciences
Series SCIENTIFIC REPORTS
Type Text
Extent 10 pages
Language English
Identifier DOI: 10.1038/s41598-017-11134-y / ISSN: 2045-2322
Rights
Citation Zheng, T., Li, H., Han, N., Wang, S., Price, J. H., Wang, M., & Zhang, D. (2017). Functional Characterization of Two Elongases of Very Long-Chain Fatty Acid from Tenebrio molitor L. (Coleoptera: Tenebrionidae). Scientific Reports, 7(1). doi:10.1038/s41598-017-11134-y
Note The final version of this article, as published in Scientific Reports, can be viewed online at: http://www.nature.com/articles/s41598-017-11134-y
Collaborating Institutions ASU Libraries
Additional Formats MODS / OAI Dublin Core / RIS


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