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Low Base-Substitution Mutation Rate in the Germline Genome of the Ciliate Tetrahymena thermophil

Abstract Mutation is the ultimate source of all genetic variation and is, therefore, central to evolutionary change. Previous work on Paramecium tetraurelia found an unusually low germline base-substitution mutation rate in this ciliate. Here, we tested the generality of this result among ciliates using Tetrahymena thermophila. We sequenced the genomes of 10 lines of T. thermophila that had each undergone approximately 1,000 generations of mutation accumulation (MA). We applied an existing mutation-calling pipeline and developed a new probabilistic mutation detection approach that directly models the design of an MA experiment and accommodates the noise introduced by mismapped reads. Our probabilistic mutation-calling method provides a straightforwa... (more)
Created Date 2016-09-15
Contributor Long, Hongan (Author) / Winter, David (ASU author) / Chang, Allan Y.-C. (Author) / Sung, Way (Author) / Wu, Steven (ASU author) / Balboa, Mariel (Author) / Azevedo, Ricardo B. R. (Author) / Cartwright, Reed (ASU author) / Lynch, Michael (Author) / Zufall, Rebecca A. (Author) / Biodesign Institute / Center for Evolution and Medicine / College of Liberal Arts and Sciences / School of Life Sciences
Type Text
Extent 11 pages
Language English
Identifier DOI: 10.1093/gbe/evw223 / ISSN: 1759-6653
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Citation Long, H., Winter, D. J., Chang, A. Y., Sung, W., Wu, S. H., Balboa, M., . . . Zufall, R. A. (2016). Low Base-Substitution Mutation Rate in the Germline Genome of the CiliateTetrahymena thermophil. Genome Biology and Evolution. doi:10.1093/gbe/evw223
Note The final version of this article, as published in Genome Biology and Evolution, can be viewed online at:
Collaborating Institutions ASU Library
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