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Antigen 85B peptidomic analysis allows species-specific mycobacterial identification

Abstract Background
Nontuberculous mycobacteria (NTM)-mediated infections are a growing cause of worldwide morbidity, but lack of rapid diagnostics for specific NTM species can delay the initiation of appropriate treatment regimens. We thus examined whether mass spectrometry analysis of an abundantly secreted mycobacterial antigen could identify specific NTM species.
We analyzed predicted tryptic peptides of the major mycobacterial antigen Ag85B for their capacity to distinguish Mycobacterium tuberculosis and three NTM species responsible for the majority of pulmonary infections caused by slow-growing mycobacterial species. Next, we analyzed trypsin-digested culture supernatants of these four mycobacterial species by liquid chromatography–ta... (more)
Created Date 2018-01-08
Contributor Zhang, Wei (Author) / Shu, Qingbo (ASU author) / Zhao, Zhen (Author) / Fan, Jia (ASU author) / Lyon, Christopher (ASU author) / Zelazny, Adrian M. (Author) / Hu, Ye (ASU author) / Biodesign Institute / Personalized Diagnostics / Ira A. Fulton Schools of Engineering / School of Biomedical and Health Systems Engineering
Type Text
Extent 10 pages
Language English
Identifier DOI: 10.1186/s12014-017-9177-6 / ISSN: 1542-6416 / ISSN: 1559-0275
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Citation Zhang, W., Shu, Q., Zhao, Z., Fan, J., Lyon, C. J., Zelazny, A. M., & Hu, Y. (2018). Antigen 85B peptidomic analysis allows species-specific mycobacterial identification. Clinical Proteomics, 15(1). doi:10.1186/s12014-017-9177-6
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