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Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimer's disease (ASUi003-A) and non-demented control (ASUi004-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant


Abstract Although the majority of late-onset Alzheimer's disease (AD) patients are labeled sporadic, multiple genetic risk variants have been identified, the most powerful and prevalent of which is the e4 variant of the Apolipoprotein E (APOE) gene. Here, we generated human induced pluripotent stem cell (hiPSC) lines from the peripheral blood mononuclear cells (PBMCs) of a clinically diagnosed AD patient [ASUi003-A] and a non-demented control (NDC) patient [ASUi004-A] homozygous for the APOE4 risk allele. These hiPSCs maintained their original genotype, expressed pluripotency markers, exhibited a normal karyotype, and retained the ability to differentiate into cells representative of the three germ layers.
Created Date 2017-07-10
Contributor Brookhouser, Nicholas (ASU author) / Zhang, Ping (Author) / Caselli, Richard (Author) / Kim, Jean J. (Author) / Brafman, David (ASU author) / Ira A. Fulton Schools of Engineering / School of Biomedical and Health Systems Engineering
Series STEM CELL RESEARCH
Type Text
Extent 4 pages
Language English
Identifier DOI: 10.1016/j.scr.2017.07.003 / ISSN: 1873-5061 / ISSN: 1876-7753
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Citation Brookhouser, N., Zhang, P., Caselli, R., Kim, J. J., & Brafman, D. A. (2017). Generation and characterization of human induced pluripotent stem cell (hiPSC) lines from an Alzheimers disease (ASUi003-A) and non-demented control (ASUi004-A) patient homozygous for the Apolipoprotein e4 (APOE4) risk variant. Stem Cell Research, 25, 266-269. doi:10.1016/j.scr.2017.07.003
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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