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Laser-fabricated cell patterning stencil for single cell analysis


Abstract Precise spatial positioning and isolation of mammalian cells is a critical component of many single cell experimental methods and biological engineering applications. Although a variety of cell patterning methods have been demonstrated, many of these methods subject cells to high stress environments, discriminate against certain phenotypes, or are a challenge to implement. Here, we demonstrate a rapid, simple, indiscriminate, and minimally perturbing cell patterning method using a laser fabricated polymer stencil. The stencil fabrication process requires no stencil-substrate alignment, and is readily adaptable to various substrate geometries and experiments.
Created Date 2017-12-19
Contributor Messner, Jacob J. (Author) / Glenn, Honor (ASU author) / Meldrum, Deirdre (ASU author) / Biodesign Institute / Center for Biosignatures Discovery Automation
Series BMC BIOTECHNOLOGY
Type Text
Extent 9 pages
Language English
Identifier DOI: 10.1186/s12896-017-0408-8 / ISSN: 1472-6750
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Citation Messner, J. J., Glenn, H. L., & Meldrum, D. R. (2017). Laser-fabricated cell patterning stencil for single cell analysis. BMC Biotechnology, 17(1). doi:10.1186/s12896-017-0408-8
Note The electronic version of this article is the complete one and can be found online at: https://bmcbiotechnol.biomedcentral.com/articles/10.1186/s12896-017-0408-8
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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