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Multiplexed Nucleic Acid Programmable Protein Arrays


Abstract Rationale: Cell-free protein microarrays display naturally-folded proteins based on just-in-time in situ synthesis, and have made important contributions to basic and translational research. However, the risk of spot-to-spot cross-talk from protein diffusion during expression has limited the feature density of these arrays.
Methods: In this work, we developed the Multiplexed Nucleic Acid Programmable Protein Array (M-NAPPA), which significantly increases the number of displayed proteins by multiplexing as many as five different gene plasmids within a printed spot.
Results: Even when proteins of different sizes were displayed within the same feature, they were readily detected using protein-specific antibodies. Protein-protein interactions a... (more)
Created Date 2017-09-20
Contributor Yu, Xiaobo (Author) / Song, Lusheng (ASU author) / Petritis, Brianne (ASU author) / Bian, Xiaofang (ASU author) / Wang, Haoyu (ASU author) / Viloria, Jennifer (ASU author) / Park, Jin (ASU author) / Bui, Hoang (ASU author) / Li, Han (ASU author) / Wang, Jie (ASU author) / Liu, Lei (Author) / Yang, Liuhui (Author) / Duan, Hu (Author) / McMurray, David N. (Author) / Achkar, Jacqueline M. (Author) / Magee, Mitch (ASU author) / Qiu, Ji (ASU author) / LaBaer, Joshua (ASU author) / Biodesign Institute / Personalized Diagnostics
Series THERANOSTICS
Type Text
Extent 14 pages
Language English
Identifier DOI: 10.7150/thno.20151 / ISSN: 1309-1042
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Citation Yu, X., Song, L., Petritis, B., Bian, X., Wang, H., Viloria, J., . . . Labaer, J. (2017). Multiplexed Nucleic Acid Programmable Protein Arrays. Theranostics, 7(16), 4057-4070. doi:10.7150/thno.20151
Note Copyright Ivyspring International Publisher
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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