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Nonsense-Mediated RNA Decay Influences Human Embryonic Stem Cell Fate


Abstract Nonsense-mediated RNA decay (NMD) is a highly conserved pathway that selectively degrades specific subsets of RNA transcripts. Here, we provide evidence that NMD regulates early human developmental cell fate. We found that NMD factors tend to be expressed at higher levels in human pluripotent cells than in differentiated cells, raising the possibility that NMD must be downregulated to permit differentiation. Loss- and gain-of-function experiments in human embryonic stem cells (hESCs) demonstrated that, indeed, NMD downregulation is essential for efficient generation of definitive endoderm. RNA-seq analysis identified NMD target transcripts induced when NMD is suppressed in hESCs, including many encoding signaling components. This led us to ... (more)
Created Date 2016-06-14
Contributor Lou, Chih-Hong (Author) / Dumdie, Jennifer (Author) / Goetz, Alexandra (Author) / Shum, Eleen Y. (Author) / Brafman, David (ASU author) / Liao, Xiaoyan (Author) / Mora-Castilla, Sergio (Author) / Ramaiah, Madhuvanthi (Author) / Cook-Andersen, Heidi (Author) / Laurent, Louise (Author) / Wilkinson, Miles F. (Author) / Ira A. Fulton Schools of Engineering / School of Biomedical and Health Systems Engineering
Series STEM CELL REPORTS
Type Text
Extent 14 pages
Language English
Identifier DOI: 10.1016/j.stemcr.2016.05.008 / ISSN: 2213-6711
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Citation Lou, C., Dumdie, J., Goetz, A., Shum, E. Y., Brafman, D., Liao, X., . . . Wilkinson, M. F. (2016). Nonsense-Mediated RNA Decay Influences Human Embryonic Stem Cell Fate. Stem Cell Reports, 6(6), 844-857. doi:10.1016/j.stemcr.2016.05.008
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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