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Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells


Abstract Quantitative three-dimensional (3D) computed tomography (CT) imaging of living single cells enables orientation-independent morphometric analysis of the intricacies of cellular physiology. Since its invention, x-ray CT has become indispensable in the clinic for diagnostic and prognostic purposes due to its quantitative absorption-based imaging in true 3D that allows objects of interest to be viewed and measured from any orientation. However, x-ray CT has not been useful at the level of single cells because there is insufficient contrast to form an image. Recently, optical CT has been developed successfully for fixed cells, but this technology called Cell-CT is incompatible with live-cell imaging due to the use of stains, such as hematoxylin... (more)
Created Date 2017-12-06
Contributor Kelbauskas, Laimonas (ASU author) / Shetty, Rishabh Manoj (ASU author) / Cao, Bin (ASU author) / Wang, Kuo-Chen (ASU author) / Smith, Dean (ASU author) / Wang, Hong (ASU author) / Chao, Shi-Hui (ASU author) / Gangaraju, Sandhya (ASU author) / Ashcroft, Brian (ASU author) / Kritzer, Margaret (ASU author) / Glenn, Honor (ASU author) / Johnson, Roger (ASU author) / Meldrum, Deirdre (ASU author) / Biodesign Institute / Center for Biosignatures Discovery Automation
Series SCIENCE ADVANCES
Type Text
Extent 15 pages
Language English
Identifier DOI: 10.1126/sciadv.1602580 / ISSN: 2375-2548
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Citation Kelbauskas, L., Shetty, R., Cao, B., Wang, K., Smith, D., Wang, H., . . . Meldrum, D. R. (2017). Optical computed tomography for spatially isotropic four-dimensional imaging of live single cells. Science Advances, 3(12). doi:10.1126/sciadv.1602580
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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