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Peptide Sequencing Directly on Solid Surfaces Using MALDI Mass Spectrometry


Abstract There are an increasing variety of applications in which peptides are both synthesized and used attached to solid surfaces. This has created a need for high throughput sequence analysis directly on surfaces. However, common sequencing approaches that can be adapted to surface bound peptides lack the throughput often needed in library-based applications. Here we describe a simple approach for sequence analysis directly on solid surfaces that is both high speed and high throughput, utilizing equipment available in most protein analysis facilities. In this approach, surface bound peptides, selectively labeled at their N-termini with a positive charge-bearing group, are subjected to controlled degradation in ammonia gas, resulting in a set of f... (more)
Created Date 2017-12-19
Contributor Zhao, Zhan-Gong (ASU author) / Cordovez, Lalaine Anne (ASU author) / Johnston, Stephen (ASU author) / Woodbury, Neal (ASU author) / Biodesign Institute / Innovations in Medicine / College of Liberal Arts and Sciences / School of Molecular Sciences
Series SCIENTIFIC REPORTS
Type Text
Extent 8 pages
Language English
Identifier DOI: 10.1038/s41598-017-18105-3 / ISSN: 2045-2322
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Citation Zhao, Z., Cordovez, L. A., Johnston, S. A., & Woodbury, N. (2017). Peptide Sequencing Directly on Solid Surfaces Using MALDI Mass Spectrometry. Scientific Reports, 7(1). doi:10.1038/s41598-017-18105-3
Note The final version of this article, as published in Scientific Reports, can be viewed online at: http://www.nature.com/articles/s41598-017-18105-3
Collaborating Institutions ASU Library
Additional Formats MODS / OAI Dublin Core / RIS


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